Contact an Account ManagerSimple and rapid micromethod for the determination of levodopa and 3-O-methyldopa in human plasma by HPLC with coulometric detection
Summary
Type: HPLC
Application #: 107660
Catalog#: 720014.40 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4x250mm
Detection: electrochemical, conditioning cell +0.35V, analytical cell with dual coulometric electrodes at +0.04V and -0.30V, respectively, A) - C) 500nA full scale, D) 50nA full scale
Conditions: eluent: phosphate / acetate buffer - acetonitrile ((87.5:12.5)
flow rate 1.0ml/min
pressure 13.8MPa
Authors: Baruzzi A
Source: J Chromatogr 375 (1986) 165 - 169
Details
Legend: Fig. 1. Chromatograms of A) deproteinized blank plasma, B) standard mixture of
6.3µmol/l levodopa and 22.0µmol/l OMD, C) deproteinized patient's plasma
(2.8µmol/l levodopa, 11.0µmol/l OMD), and D) d